Василиса▶ Я жду вашего обращения. Что Вы хотите узнать?
Arenavirus - Wikipedia ArenavirusFrom Wikipedia, the free encyclopedia
Lassa virus virions TEM 8699 lores.jpg
Lassa virus
Virus classification e
(unranked): Virus
Realm : Riboviria
Phylum: Negarnaviricota
Class: Ellioviricetes
Order: Bunyavirales
Family: Arenaviridae

An arenavirus is a negative-sense, single-stranded RNA virus that is a member of the family Arenaviridae . These viruses infect rodents and occasionally humans; arenaviruses have also been discovered which infect snakes. At least eight arenaviruses are known to cause human disease. The diseases derived from arenaviruses range in severity. Aseptic meningitis, a severe human disease that causes inflammation covering the brain and spinal cord, can arise from the lymphocytic choriomeningitis virus . Hemorrhagic fever syndromes, including Lassa fever , are derived from infections such as Guanarito virus , Junin virus , Lassa virus , Lujo virus , Machupo virus , Sabia virus , or Whitewater Arroyo virus . Arenaviruses are divided into two groups: the Old World and the New World viruses. The differences between these groups are distinguished geographically and genetically. Because of the epidemiological association with rodents, some arenaviruses and bunyaviruses are designated as roboviruses .

ContentsStructure[ edit ]

Viewed in cross-section, arenaviruses contain grainy particles that are ribosomes acquired from their host cells. It is from this characteristic that they acquired the name arena , from the Latin root meaning sand . The ribosomal structures are not believed to be essential for virus replication. Virus particles, or virions, are pleomorphic (variable in shape) but are often spherical, with a diameter of 60–300 nm, and are covered with surface glycoprotein spikes.

The virus contains a beaded nucleocapsid with two single-stranded RNA segments. The nucleocapsid consists of a core of nucleic acid enclosed in a protein coat. Although they are categorized as negative-sense viruses, arenaviruses are ambisense . While sections of their genome encode genes in the negative sense (reverse polarity), other sections encode genes in the opposite (forward/positive sense) direction. This complex gene expression structure is theorized to be a primitive regulatory system, allowing the virus to control what proteins are synthesized at what point in the life cycle. The life cycle of the arenavirus is restricted to the cell cytoplasm.

Taxonomy[ edit ]

Within the family Arenaviridae , arenaviruses were formerly all placed in the genus Arenavirus , but were recently (2014) reclassified into the genera Mammarenavirus for those with mammalian hosts and Reptarenavirus for those infecting snakes.

A third genus - Hartmanivirus - has also been established. Species in the Hartmanivirus also infect snakes. The organisation of the genome of this genus is typical of arenaviruses but their glycoproteins resemble those of filoviruses . Species in this genus lack the matrix protein.

An arenavirus - Wenling frogfish arenavirus - has been described in fish. Its classification has yet to be decided.

Arenaviruses can be divided into two serogroups, which differ genetically and by geographical distribution: When the virus is classified “Old World” this means it was found in the Eastern Hemisphere in places such as Europe, Asia, and Africa. When it is found in the Western Hemisphere, in places such as Argentina, Bolivia, Venezuela, Brazil, and the United States, it is classified “New World”. Lymphocytic choriomeningitis (LCM) virus is the only arenavirus to exist in both areas but is classified as an Old World virus.

Old and New World area viruses appear to have diverged ~45,000 years ago. The Old World Mammarenaviruses originated ∼23.1-1.88 thousand years ago, most likely in Southern Africa while the New World Mammarenaviruses evolved in the Latin America-Caribbean region ∼41.4-3.3 thousand years ago.

Mammarenavirus[ edit ]Old World complex: Dandenong virus Gairo virus Gbagroube virus Ippy virus Kodoko virus Lassa virus Loei River virus Lujo virus Luna virus Lunk virus Lymphocytic choriomeningitis virus Mariental virus Merino Walk virus Menekre virus Minu virus Mobala virus Morogoro virus Mopeia virus Okahandja virus Pichinde virus Pirital virus Wenzhou virus Cardamones virus New World complex:Clade A Allpahuayo virus Flexal virus Paraná virus Pichindé virus Pirital virus Clade B Amaparí virus Chapare virus Cupixi virus Guanarito virus Junín virus Machupo virus Tacaribe virus Big Brushy Tank virus (BBTV) Ocozocoautla de Espinosa virus Real de Catorce virus (RCTV) Rio Catorce virus Tonto Creek virus (TTCV) Xapuri virus Sabiá virus Clade C Latino virus Oliveros virus Clade D Bear Canyon virus Catarina virus (CTNV) Skinner Tank virus (SKTV) Tamiami virus Whitewater Arroyo virus Others Patawa virus Reptarenavirus[ edit ] CAS virus California Academy of Sciences virus Collierville virus Golden Gate virus University of Helsinki virus Hartmanivirus[ edit ] Dante Muikkunen virus Haartman hartmanivirus Old Schoolhouse virus Veterinary Pathology Zurich virus Unclassified[ edit ] Wenling frogfish arenavirus Genome[ edit ]

Arenaviruses have a segmented RNA genome that consists of two single-stranded ambisense RNAs. As with all negative-sense RNA viruses, the genomic RNA alone is not infectious and the viral replication machinery is required to initiate infection within a host cell. Genomic sense RNA packaged into the arenavirus virion is designated negative-sense RNA, and must first be copied into a positive-sense mRNA in order to produce viral protein . The two RNA segments are denoted Small (S) and Large (L), and code for four viral proteins in a unique ambisense coding strategy. Each RNA segment codes for two viral proteins in opposite orientation such that the negative-sense RNA genome serves as the template for transcription of a single mRNA and the positive-sense copy of the RNA genome templates a second mRNA . The separate coding sequences of the two viral proteins are divided by an intergenic region RNA sequence that is predicted to fold into a stable hairpin structure.

The extreme termini of each RNA segment contains a 19 nucleotide highly conserved sequence that is critical for recruitment of the viral replication machinery and initiation of viral mRNA transcription and genomic replication . The conserved 5' and 3' RNA termini sequences are complementary and allows each RNA segment to adopt a double-stranded RNA panhandle structure that maintains the termini in close proximity and results in a circular appearance to purified arenavirus genomic templates visualized by electron microscopy . The double-stranded RNA panhandle structure is critical for efficient viral RNA synthesis, but potential interterminal double-stranded RNA interactions must be transiently relieved in order to recruit the viral polymerase .

The S-segment RNA is approximately 3.5 kb, and encodes the viral nucleocapsid protein (NP) and glycoprotein (GPC). The L-segment RNA is approximately 7.2 kb, and encodes the viral RNA-dependent RNA-polymerase (L) and a small RING-domain containing protein (Z).

The Z protein forms homo oligomers and a structural component of the virions. The formation of these oligomers is an essential step for particle assembly and budding. Binding between Z and the viral envelope glycoprotein complex is required for virion infectivity. Z also interacts with the L and NP proteins. Polymerase activity appears to be modulated by the association between the L and Z proteins. Interaction between the Z and NP proteins is critical for genome packaging.

Evolution[ edit ]

The evolution of the Mammarenavirus genus has been studied. The New World and Old World species diverged less than 45,000 years ago. The New World species evolved between 41,400 and 3,300 years ago in the Latin America-Caribbean region. The Old World species evolved between 23,100 and 1,880 years ago, most likely in southern Africa.

Molecular biology[ edit ]

The glycoprotein (GP) is synthesised as a precursor molecule. It is cleaved into three parts - GP1, GP2 and a stable signal peptide (SSP). These reactions are catalysed by cellular signal peptidases and the cellular enzyme Subtilisin Kexin Isozyme-1 (SKI-1)/Site-1 Protease (S1P). These processes are essential for fusion competence and incorporation of mature GP into nascent budding virion particles.

Reservoirs[ edit ]

Some arenaviruses are zoonotic pathogens and are generally associated with rodent —transmitted disease in humans. Each virus usually is associated with a particular rodent host species in which it is maintained. Arenaviruses persist in nature by infecting rodents first and then transmitted into humans. Humans can be infected through mucosal exposure to aerosols, or by direct contact of abraded skin with the infectious material, derived from infected rodents. Aerosols are fine mists or sprays of rodent dried excreta, especially urine that is dropped in the environment. Most of the Arenaviruses caught by humans are within their own homes when these rodents seek shelter. The virus can be caught in factories, from food that has been contaminated, or within agricultural work areas. Humans' risk of contracting the Arenavirus infection is related to age, race, or sex within the degree of contact with the dried rodent excreta.

Epidemiology[ edit ]Hosts[ edit ]
Arenavirus diseases and hosts
Virus Disease Host Distribution
Lymphocytic choriomeningitis virus Lymphocytic choriomeningitis House mouse ( Mus musculus ) Worldwide
Lassa virus Lassa fever Natal Multimammate Mouse ( Mastomys natalensis ) West Africa
Junin virus Argentine hemorrhagic fever Drylands Vesper Mouse ( Calomys musculinus ) Argentina
Machupo virus Bolivian hemorrhagic fever Large Vesper Mouse ( Calomys callosus ) Bolivia
Guanarito virus Venezuelan hemorrhagic fever Short-tailed Cane Mouse ( Zygodontomys brevicauda ) Venezuela
Sabiá virus Brazilian hemorrhagic fever Unknown Brazil
Tacaribe virus Bat ( Artibeus ) Trinidad
Flexal virus Influenza -like illness Rice rat ( Oryzomys ) Brazil
Whitewater Arroyo virus Hemorrhagic fever Woodrat ( Neotoma ) Southwestern United States
Clinical diseases[ edit ]LCM viruses cause influenza -like febrile illness, but occasionally they may cause meningitis, characteristically accompanied by large numbers of lymphocytes in the CSF (as the name LCM suggests).Lassa fever virus causes Lassa fever. Lassa fever is endemic in west Africa.The virus was first isolated from Americans stationed in the village of Lassa, Nigeria.The virus can be transmitted person-to -person. Subclinical diseases: Serological studies suggest that inapparent infections particularly among members of hunting tribes are common.Clinical infections:Lassa fever is characterised by high fever, severe myalgia, coagulopathy, haemorrhagic skin rash, and occasional visceral haemorrhage as well as necrosis of liver and spleen.Other Arenaviruses like Junin virus, Machupo virus cause haemorrhagic fevers.

All of these diseases pose a great threat to public health in the regions where it is taking place. For example, when the Old World Lassa virus turns into Lassa fever, this usually results in a significant amount of mortality. Similarly the New World Junin virus causes Argentine hemorrhagic fever. This fever is a several illness with hemorrhagic and neurological manifestations and a case fatality of fifteen to thirty percent. The way this virus spreads is through increased traveling to and from endemic regions. This traveling has led to the importation of Lassa fever into non-endemic metropolitan areas all over the world.

Recent outbreaks[ edit ]

A new species of arenavirus named the Lujo virus has been linked to five patients who exhibited symptoms of viral hemorrhagic fever in South Africa. The disease originated near Lusaka, Zambia and spread to Johannesburg , South Africa , after the first patient was transported to a hospital there. The results of genetic sequencing tests conducted by epidemiologists at Columbia University in New York City , USA, and at the Special Pathogens Branch of the Centers for Disease Control in Atlanta , USA, provided evidence that the causative agent of the disease is a virus from the family Arenaviridae, which ultimately resulted in the deaths of four out of the five infected in Zambia and South Africa during the outbreak which began in September 2008.

Arenavirus has also recently pinpointed as the cause of death of three donor organ recipients in Australia who contracted the virus after receiving kidney and a liver donations from a single infected organ donor in late 2006. All three died in the first week of 2007.


WHO and its Global Outbreak Alert and Response Network (GOARN) partners continue to support the Ministries of Health of the two countries in various facets of the outbreak investigation, including laboratory diagnosis, investigations, active case finding and follow-up of contacts.

Treatments[ edit ]

This virus can be very devastating yet there are very few treatment methods available. The current lack of a licensed vaccine and limited therapeutic options for the Arenavirus make it arguably among the most neglected virus to be dealt with. The only licensed drug for the treatment of human Arenavirus infection is the nucleoside analogue ribavirin. Ribavirin reduces morbidity and mortality in humans who have certain Arenaviruses, such as LASV and JUNV infections, if it is taken in the early stages of the disease. Ribavirin displays mixed success in treating severe Arenaviral disease and is associated with significant toxicities. Effective anti-viral drugs need to be produced at a low cost, taken orally, and able to withstand tropical climates due to the regions where these infections are occurring. For this reason high throughput screening (HTS) of small molecular libraries could be the answer to finding a better remedy. HTS collects libraries of small synthetic molecules that can be used to identify protein promoting “agonist” molecules or protein inhibiting “antagonist” interactions. With HTS sustainable anti-viral drugs can be discovered against possible new human pathogenic viruses.

References[ edit ]Radoshitzky, SR; Buchmeier, MJ; Charrel, RN; Clegg, JCS; Gonzalez, JJ; Günther, S; Hepojoki, J; Kuhn, JH; Lukashevich, IS; Romanowski, V; Salvato, MS; Sironi, M; Stenglein, MD; de la Torre, JC; ICTV Report, Consortium (August 2019). "ICTV Virus Taxonomy Profile: Arenaviridae". The Journal of General Virology. 100 (8): 1200–1201. doi : 10.1099/jgv.0.001280 . PMID   31192784 . Briese T, Paweska JT, McMullan LK, et al. (May 2009). "Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa" . PLoS Pathog. 5 (5): e1000455. doi : 10.1371/journal.ppat.1000455 . PMC   2680969 . PMID   19478873 .Botten, J; Whitton, JL; Barrowman, P; Sidney, J; Whitmire, JK; Alexander, J; Kotturi, MF; Sette, A; Buchmeier, MJ (2010). "A Multivalent Vaccination Strategy for the Prevention of Old World Arenavirus Infection in Humans" . Journal of Virology. 84 (19): 9947–56. doi : 10.1128/JVI.00672-10 . PMC   2937778 . PMID   20668086 .^ Emonet, Sebastien E.; Urata, Shuzo; De La Torre, Juan C. (2011). "Arenavirus reverse genetics: New approaches for the investigation of arenavirus biology and development of antiviral strategies" . Virology. 411 (2): 416–425. doi : 10.1016/j.virol.2011.01.013 . PMC   3057228 . PMID   21324503 . "Arenaviridae - Negative Sense RNA Viruses - Negative Sense RNA Viruses (2011)" . International Committee on Taxonomy of Viruses (ICTV). Retrieved 23 May 2019. ICTV proposals 2014.011a-dV et al. Archived 4 March 2016 at the Wayback Machine , Mark D. Stenglein et al. ICTV proposals 2014.012aV et al. Archived 4 March 2016 at the Wayback Machine , Michael J. Buchmeier et al.Stenglein MD, Sanders C, Kistler AL, et al. (2012). "Identification, characterization, and in vitro culture of highly divergent arenaviruses from boa constrictors and annulated tree boas: candidate etiological agents for snake inclusion body disease" . mBio. 3 (4): e00180–12. doi : 10.1128/mBio.00180-12 . PMC   3419519 . PMID   22893382 .Zhang YZ, Wu WC, Shi M, Holmes EC (2018) The diversity, evolution and origins of vertebrate RNA viruses. Curr Opin Virol 31:9-16Delgado S, Erickson BR, Agudo R, et al. (April 2008). "Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia" . PLoS Pathog. 4 (4): e1000047. doi : 10.1371/journal.ppat.1000047 . PMC   2277458 . PMID   18421377 .^ Forni, D; Pontremoli, C; Pozzoli, U; Clerici, M; Cagliani, R; Sironi, M (1 March 2018). "Ancient evolution of Mammarenaviruses: Adaptation via changes in the L protein and no evidence for host-virus codivergence" . Genome Biology and Evolution. 10 (3): 863–874. doi : 10.1093/gbe/evy050 . PMC   5863214 . Retrieved 16 February 2019.^ Añón MC, Grau O, Segovia ZM, Franzefernández MT (June 1976). "RNA composition of Junin virus" . J. Virol. 18 (3): 833–8. PMC   354781 . PMID   178925 .Lee KJ, Novella IS, Teng MN, Oldstone MB, de La Torre JC (April 2000). "NP and L proteins of lymphocytic choriomeningitis virus (LCMV) are sufficient for efficient transcription and replication of LCMV genomic RNA analogs" . J. Virol. 74 (8): 3470–7. doi : 10.1128/jvi.74.8.3470-3477.2000 . PMC   111854 . PMID   10729120 .^ Meyer BJ, de la Torre JC, Southern PJ (2002). "Arenaviruses: genomic RNAs, transcription, and replication". Curr. Top. Microbiol. Immunol. 262: 139–57. PMID   11987804 .Pedersen IR (March 1973). "Different classes of ribonucleic acid isolated from lymphocytic choriomeningitis virus" . J. Virol. 11 (3): 416–23. PMC   355116 . PMID   4734917 .Auperin DD, Romanowski V, Galinski M, Bishop DH (December 1984). "Sequencing studies of pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA" . J. Virol. 52 (3): 897–904. PMC   254611 . PMID   6492264 .Auperin DD, McCormick JB (February 1989). "Nucleotide sequence of the Lassa virus (Josiah strain) S genome RNA and amino acid sequence comparison of the N and GPC proteins to other arenaviruses". Virology. 168 (2): 421–5. doi : 10.1016/0042-6822(89)90287-0 . PMID   2916333 .Auperin DD, Galinski M, Bishop DH (April 1984). "The sequences of the N protein gene and intergenic region of the S RNA of pichinde arenavirus". Virology. 134 (1): 208–19. doi : 10.1016/0042-6822(84)90286-1 . PMID   6324469 .Auperin D, Dimock K, Cash P, Rawls WE, Leung WC, Bishop DH (January 1982). "Analyses of the genomes of prototype pichinde arenavirus and a virulent derivative of Pichinde Munchique: evidence for sequence conservation at the 3' termini of their viral RNA species". Virology. 116 (1): 363–7. doi : 10.1016/0042-6822(82)90429-9 . PMID   6278715 .Auperin DD, Compans RW, Bishop DH (August 1982). "Nucleotide sequence conservation at the 3' termini of the virion RNA species of New World and Old World arenaviruses". Virology. 121 (1): 200–3. doi : 10.1016/0042-6822(82)90130-1 . PMID   6287720 .Mattsson L (1989). "Chronic non-A, non-B hepatitis with special reference to the transfusion-associated form". Scand J Infect Dis Suppl. 59: 1–55. PMID   2502835 .^ Hass M, Westerkofsky M, Müller S, Becker-Ziaja B, Busch C, Günther S (December 2006). "Mutational analysis of the lassa virus promoter" . J. Virol. 80 (24): 12414–9. doi : 10.1128/JVI.01374-06 . PMC   1676312 . PMID   17005649 .^ Kranzusch PJ, Schenk AD, Rahmeh AA, et al. (November 2010). "Assembly of a functional Machupo virus polymerase complex" . Proc. Natl. Acad. Sci. U.S.A. 107 (46): 20069–74. doi : 10.1073/pnas.1007152107 . PMC   2993349 . PMID   20978208 .Bishop DH, Auperin DD (1987). "Arenavirus gene structure and organization". Curr. Top. Microbiol. Immunol. 133: 5–17. PMID   2435460 .Young PR, Howard CR (April 1983). "Fine structure analysis of Pichinde virus nucleocapsids". J. Gen. Virol. 64 (4): 833–42. doi : 10.1099/0022-1317-64-4-833 . PMID   6682139 .Palmer EL, Obijeski JF, Webb PA, Johnson KM (September 1977). "The circular, segmented nucleocapsid of an arenavirus-Tacaribe virus". J. Gen. Virol. 36 (3): 541–5. doi : 10.1099/0022-1317-36-3-541 . PMID   199698 .Perez M, de la Torre JC (January 2003). "Characterization of the genomic promoter of the prototypic arenavirus lymphocytic choriomeningitis virus" . J. Virol. 77 (2): 1184–94. doi : 10.1128/jvi.77.2.1184-1194.2003 . PMC   140842 . PMID   12502835 .Ghiringhelli PD, Rivera-Pomar RV, Lozano ME, Grau O, Romanowski V (September 1991). "Molecular organization of Junin virus S RNA: complete nucleotide sequence, relationship with other members of the Arenaviridae and unusual secondary structures". J. Gen. Virol. 72 (9): 2129–41. CiteSeerX . doi : 10.1099/0022-1317-72-9-2129 . PMID   1654373 .Iapalucci S, López N, Rey O, Zakin MM, Cohen GN, Franze-Fernández MT (November 1989). "The 5' region of Tacaribe virus L RNA encodes a protein with a potential metal binding domain". Virology. 173 (1): 357–61. doi : 10.1016/0042-6822(89)90257-2 . PMID   2510403 .Iapalucci S, Lopez R, Rey O, et al. (May 1989). "Tacaribe virus L gene encodes a protein of 2210 amino acid residues". Virology. 170 (1): 40–7. doi : 10.1016/0042-6822(89)90349-8 . PMID   2718387 .Salvato MS, Shimomaye EM (November 1989). "The completed sequence of lymphocytic choriomeningitis virus reveals a unique RNA structure and a gene for a zinc finger protein". Virology. 173 (1): 1–10. doi : 10.1016/0042-6822(89)90216-X . PMID   2510401 .Loureiro ME, D'Antuono A, Levingston Macleod JM, López N (September 2012). "Uncovering viral protein-protein interactions and their role in arenavirus life cycle" . Viruses. 4 (9): 1651–67. doi : 10.3390/v4091651 . PMC   3499824 . PMID   23170177 .Burri DJ, da Palma JR, Kunz S, Pasquato A (October 2012). "Envelope glycoprotein of arenaviruses" . Viruses. 4 (10): 2162–81. doi : 10.3390/v4102162 . PMC   3497046 . PMID   23202458 . Scientists identify new lethal virus in Africa "Virus identified - nurse ill" . News24.com. Archived from the original on 13 October 2008. Retrieved 13 October 2008. "Virus kills organ recipients" . www.theage.com.au. Retrieved 16 October 2009.^ Lee, A. M.; Pasquato, A.; Kunz, S. (2011). "Novel approaches in anti-arenaviral drug development" . Virology. 411 (2): 163–169. doi : 10.1016/j.virol.2010.11.022 . PMC   3057354 . PMID   21183197 .Mendenhall, M.; Russell, A.; Juelich, T.; Messina, E. L.; Smee, D. F.; Freiberg, A. N.; Holbrook, M. R.; Furuta, Y.; et al. (2010). "T-705 (Favipiravir) Inhibition of Arenavirus Replication in Cell Culture" . Antimicrobial Agents and Chemotherapy. 55 (2): 782–787. doi : 10.1128/AAC.01219-10 . PMC   3028760 . PMID   21115797 .External links[ edit ] ICTV Report: Arenaviridae Viralzone : Arenavirus Detailed genomic and bioinformatic information about Arenaviridae at NIH-funded database . Arenaviridae Genomes database search results from the Viral Bioinformatics Resource Center . Google.Org blog info on recent outbreak. Virus Pathogen Database and Analysis Resource (ViPR): Arenaviridae Arenaviruses " Arenavirus " . NCBI Taxonomy Browser. 11618. Retrieved from " https://en.wikipedia.org/w/index.php?title=Arenavirus&oldid=926369553 " Categories : Arthropod-borne viral fevers and viral haemorrhagic fevers Arenaviridae Hidden categories: Webarchive template wayback links Use dmy dates from April 2017 Articles with 'species' microformats Navigation menuPersonal toolsNot logged in Talk Contributions Create account Log in Namespaces Article Talk VariantsViews Read Edit View history MoreSearch
Navigation Main page Contents Featured content Current events Random article Donate to Wikipedia Wikipedia store Interaction Help About Wikipedia Community portal Recent changes Contact page Tools What links here Related changes Upload file Special pages Permanent link Page information Wikidata item Cite this page In other projects Wikimedia Commons Wikispecies Print/export Create a book Download as PDF Printable version Languages العربية Čeština Deutsch Español Français ������ Italiano Nederlands 日本語 Polski Português Slovenščina 中文 Edit links This page was last edited on 15 November 2019, at 22:29 (UTC).Text is available under the Creative Commons Attribution-ShareAlike License ; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy . Wikipedia® is a registered trademark of the Wikimedia Foundation, Inc. , a non-profit organization. Privacy policy About Wikipedia Disclaimers Contact Wikipedia Developers Statistics Cookie statement Mobile view Wikimedia Foundation Powered by MediaWiki
© 2014-2019 ЯВИКС - все права защищены.
Наши контакты/Карта ссылок